Comparing inhibitory effect some of probiotics and antibiotics against pathogenic bacteria isolated from injuries of military operations

Abstract

This study aimed to isolate the antibiotics-resistant bacteria causing burns and wounds infections in military operation patients, and to compare the inhibitory efficacy of probiotics with the most common effective antibiotics. For this purpose, collected a total of 52 male patients of ages from 18 to 55 years suffering   of wounds and burns infection from Military Muthanna Hospital form the period September 2019 to January 2020. Distributed between 34 of the samples were collected from wounds and 18 from burns. 27 (51.92%) from the wounds and 14 (26.92%) from the burns gave positive results for bacterial growth. A total of 61 bacterial isolates, 40 from wounds and 21 from burns, were obtained after culturing on MacConkey agar and blood agar and incubating at 37ºC for 24h. The isolates were identified by cultural, microscopic and biochemical tests and found to belong to both Gram positive and Gram-negative bacteria .The susceptibility test was performed by subjected each of the wound and burn isolates to four most common antibiotics to each isolate bacterium. Results showed that majority of the isolates were highly sensitive to Ciprofloxacin and Gentamicin. 8 of the highly antibiotic-resistant isolates were selected for treatment by two bacterial probiotics; Lactobacillus casei and L. paracasei Cultivated independently, in MRS broth (de Man, Rogosa and Sharpe broth) medium. The fermentation products were then concentrated (50, 25, and12.5) ml and the inhibitory activity of the eight most antibiotic-resistant isolates used was tested. Experiment was repeated three times and analysed with SPSS software. ANOVA Table with Tukey's Multiple Comparison Test. Results showed that despite the three-fold concentrated filtrates of both probiotics exerted good inhibitory activity against the pathogenic isolates, but L. casei filtrate was highly effective than that of L. paracasei. Moreover, L. casei filtrate was even competitor to Ciprofloxacin and Gentamicin.