Efficiency of biofertilizer from local isolate of Bradyrhizobium japonicum on growth and yield of the soybean plant (Glycine max L.)


  • Wael Mohammed Mahdi Mahdi
  • Abdul-Karem Araibi Al-kurtany College of Agriculture, Tikrit University




bio fertilizer, rhizobium, soybean, sterilization, and Seeds inoculation.


A field experiment was carried out to evaluate the biofertilizer efficiency prepared from local of Bradyrhizobium japonicum on the growth and yield of soybean plant. Glycine max L. in steriled loam soil and non-steriled. The results showed a significant increase in the studied characteristics of soybean in biofertilized treatments, the percentage was increased of biofertilized treatments compared to un- biofertilized treatments in the number of nodules, fresh and dry weight of 205.80%, 677.77% and 2250% respectively, while the root and shoot dry weight were increased of 173.84%, 149.94 %, inoculating treatments gave higher plant height , leaf area and nitrogen concentration in the shoot parts compared to treatments of non- inoculating with an increasing percentage of 30.02%, 244.04%, 27.66% respectively. The results also showed that higher number of pods, total yield and protein were obtained at biofertilized treatments compared to un- biofertilized treatments by percentage of 56.97%, 149.15%, 29.80% respectively. The results of sterilization showed that, a significant increase in; nodules freash and dry weight, shoot dry weight, leaf area, as well as total yield and protein concentration compared to non-sterile with appercentage of. 400%, 633.33%, 10.15%, 17.37%, 27.41%, 11.26% respectively. The outcomes of interaction between biofertilization and sterilization showed that the fertilized and sterilized treatment resulted in significant increase compared to the non-fertilized and sterilized treatment in all studied traits. The fertilized and steriled treatment and the fertilized and non-steriled treatment did not differ significantly in some of these traits, This finding could be attributed to efficiency of the strain used in the sterilization and its ability to compete with endogenous bacteria in infection and formation of nodules in the roots.