Evolution of gold nanoparticles as a drug against cutaneous leishmaniasis in vitro

Abstract

  This study was conducted between 13/12/2021 to 5/5/2022 in the city of Samarra, and 83 blood samples were collected for people with leishmaniasis. Serological tests were carried out and it was found that 69.87% of those infected had specific antibodies IgM and 30.13% had IgG antibodies. The results of the study showed the inhibitory efficacy of gold nanoparticles at size 0.15 on average growth of 1.56× 106 cells/ml in time 96, which inhibited the growth of the parasite since the start of the experiment at the probability level of p≤ 0.05 While the volume of 0.20 μL showed a high inhibitory effect on the growth of the Promastigote of the leishmania parasite at an average growth of 0.85×106 cells / ml in time 72 from the start of the experiment at a significant level p≤ 0.05. The highest level of inhibitory effectiveness of the growth of the Promastigote of the f of the leishmania parasite was at the volume of 0.30 μL, where the average growth became 0.61 cells / ml. in the 48th  time  at the start of the experiment and this volume is the best size to affect the growth of the parasite in the agricultural medium compared to the effect of the drug Betamethasone, which inhibited the growth of the parasite at the concentration of 0.50 μL to 0.24×106 cells / ml at the 48th time from the start of the experiment at a significant level p≤ 0.05.

The results of the study showed the inhibitory efficacy of gold nanoparticles at size 0.15 on average growth of 1.56× 10⁶ cells/ml in time 96 which inhibited the growth of the parasite from since the start of the experiment at the probability level of P < 0.05. While the volume of 0.20 μL showed high inhibitory effectiveness on the growth of the anterior phase of the  of the leishmaniasis parasite at an average growth of 0.85×10⁶ cells / ml in time 72 from the start of the experiment at a significant level P< 0.05 . The highest level of inhibitory effectiveness of the growth of the anterior phase of the f of the leishmaniasis parasite was at the volume of 0.30 μL, where the average growth became 0.61 cells / ml in the 48th time of the start of the experiment and this volume is the best size to affect the growth of the parasite in the agricultural medium compared to the effect of the drug pentosam, which inhibited the growth of the parasite at the concentration of 0.50 μL to 0.24×10⁶ cells / ml at the 48th time from the start of the experiment at a significant level P< 0.05.