The effect of immunizing laboratory rabbits infected with Giardia lamblia with antigen (O) purified from Escherichia coli on Mitotic index and some Immunoglobulins

Abstract

This study was conducted for the period from the beginning of December 2021 to February 2023, and it included a follow-up of the effect of outer membrane proteins (OMPs) purified from Escherichia coli bacteria in (20) male laboratory white rabbits, 10 of which developed infection with the Giardia lamblia parasite. And another in which the infection did not develop, but was only treated with O-antigen, and the change in each of mitotic index and immunoglobulins (IgM and IgG) was studied. The current results indicated that there was a significant increase in the mitotic index of bone marrow cells in groups of animals treated with the antigen compared with the negative control group, where the ratio was (60.20 and 55.60%), respectively, while the challenge group had a significant increase in the mitotic index compared to the positive control. The percentage was (74.80 and 61.80%), respectively. The current results showed a non-significant increase in the level of both IgM and IgG in the (O) antigen-treated group compared with the negative control group, where the IgM level was (73.35 and 66.27) pg/ml, respectively, and the IgG level was (1.38 and 1. (16) µg/ ml, respectively, and it also indicated to a non-significant increase in the level of IgM and a significant increase in the level of IgG in the challenge group compared with the positive control, and the level of IgM in the serum for both the challenge and positive control groups reached (85.98 and 78.69) pg/ml, respectively, while IgG levels were (2.01 and 1.46) µg/ml, respectively.