Evaluate of the effect of gold nanoparticles on some biochemical changes in rabbits experimentally infected with Giardiasis

Abstract

The study included conducting some biochemical tests in the blood serum of the groups under study to identify some biochemical changes associated with giardiasis, as well as those associated with treatment with prepared gold nanoparticles. Twenty adult male rabbits were used, divided into four main groups (5 rabbits in each group): Negative control group(C-) non infected, Positive control(C+) infected not treated, the groups G1 and G2 infection and treated.  groups all orally treated with parasite cysts (4 × 103 cysts /ml), G1 treated with metronidazole and G2 with gold nanoparticles.    The results of the study showed a decrease in the rate of shedding cysts from day one after treatment with metronidazole and   gold nanoparticles and continued to decline to zero on the fifth day and fourth day respectively compared with the positive control(C+).The biochemical results showed that the level of microsomal triglyceride transfer protein(MTTP)significant decreased (P≥0.05) in groups G+, G1, and G2, compared with the negative group (healthy rabbits). While the results of the analysis showed that the   level of triglycerides (TG) significantly decreased in groups G+ and G2 compared with the negative control group, while the TG level which showed no-significant deference in the G1 group. Also,the level cholesterol  in  the serum was not affected by the infection in the positive control , while increased of  significantly in the  groups (G1 and G2) compared to the negative group. High density lipoprotein(HDL) did not show any significant difference in the groups G + and G1 compared to the negative group, while   significantly increased which was treated with gold nanoparticles in G2. The results also indicated that the level of total antioxidant capacity(TAC) and malodialdhyde(MDA) in the blood  significant increased  in the parasite infection group (the positive group), and returns to the normal level after treatment (in G1 and G2). Conclusion from the results of the study gold nanoparticles showed is very effective in the treatment of Giardiasis and does not cause an increase the oxidative stress in the body.