Isolation and Identification of some Bacterial isolates causing Skin Infections in Humans and detect Their Resistance to Antibiotics

Abstract

In this study, swabs were taken from 80 patients with skin diseases including swabs from hair follicles, impetigo and boils. The patients included males and females with their ages ranging from (5-60) years from the outpatient clinic in Baghdad. The results showed that 35 (43.7%) of the isolates were Gram-positive bacteria, and 45 (56.2%) were Gram-negative bacteria. The rates of Gram-negative bacteria isolates were as follows:  22 (48.8%) isolates were P. aeruginosa, 10 (22.22%) isolates were E. coli, 7 (15.5%) isolates were Proteus mirabilis, 4 (8.8%) isolates were Klebsiella pneumoniae, and 2 (4.4%) isolates were Morganella morganii. Gram-positive bacteria rates were as follows: 14 (40%) isolates were S. aureus, 9 (25.7%) isolates were S. epidermidis, and 4 (11.4%) for each of Strept pyogenes, strept viridans and Strept agalactiae (Strept). The results showed a significant difference (P > 0.01) between infected males (68.7%) compared to females (31.2%), and the infection rate was higher in the age group 5-20 years for male patients (50.9%) and females (36%), with a significant difference (P>0.05) compared to other age groups of the same sex. The majority of the isolated S. aureus isolates showed sensitivity to vancomycin, ofloxacin, gentamicin/norfloxacin, imipenem, and meropenem, and resistance to trimethoprim, ceftriaxone, and penicillin G. On the other hand, they showed sensitivity to ofloxacin and imipenem, while they were resistant to aztreonam, trimethoprim, ceftriaxone, and ampicillin.