Green Synthesis-Based Spectrophotometric Method for the Determination of Triprolidine HCl in Pure Form and Pharmaceutical Products Using Biogenic Silver Nanoparticle

Abstract

The nanoparticles of metal are particularly intriguing in nanotechnology because of their distinct chemical characteristics. The purpose of this work was to develop a simple, low-toxicity, and inexpensive method to determine triprolidine hydrochloride (TRP) in pharmaceutical and pure dose forms. The current study uses an aqueous extract of Salvia officinalis leaves to create AgNPs in a green way. Ultraviolet-visible (UV-Vis) and infrared (IR) spectroscopic measurements were performed at the Chemistry Department, College of Science, Tikrit University, while other tests were performed in specialized laboratories in Iran between October 28, 2024, and March 5, 2025.The spectroscopic results showed that the maximum absorption of the nanoparticle-drug mixture was at 451 nm, with a strong linear relationship within the concentration range (2-28 µg/ml) and a correlation coefficient R² = 0.9985. The molar absorptivity was 1.3160 ​​× 10⁴ L/mol cm, and Sandell's significance was 0.02392 μg/cm². The detection limit was 0.1427 μg/mL, and the limit of quantitation was 0.4324 μg/mL. The results showed a recovery rate of 99.304%, with a relative standard deviation of 0.599% and a relative error of 0.255%. The method proved to be accurate and reliable when applied to various pharmaceutical dosage forms containing triprolidine hydrochloride.