Extraction collagen and Nanocollagen Preparation from Iraqi Catfish Skin Using Chitosan Physicochemical Characterization
DOI:
https://doi.org/10.54153/sjpas.2025.v7i4.1137Keywords:
Collagen, Nanocollagen, Chitosan, FTIR, UV-Vis, XRD, SEMAbstract
This study investigated the extraction and preparation of nanocollagen from Iraqi catfish (Siluriformes) skin, utilizing chitosan and sodium tripolyphosphate (STPP) as crosslinking agents. Collagen was extracted through an optimized process involving chemical pretreatment and lyophilization. Nanocollagen was characterized using analytical techniques including Fourier-transform infrared spectroscopy (FTIR), UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Through FTIR analysis, the resulting functional groups were identified and the chemical reactions were confirmed, UV analysis of collagen showed maximum absorption at 230 nm, XRD spectra confirmed the crystal structure associated with the triple helix conformation, SEM results indicate that ASC collagen appears as a soft white sponge, there is an obvious rough surface with a spherical structure, the results demonstrated significant improvements in the physicochemical properties of nanocollagen compared to raw collagen, including enhanced crystallinity, and particle uniformity. These findings suggest nanocollagen as a promising biomaterial for regenerative medicine and advanced wound care applications.
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