Synthesis of silver nanoparticles using green method to studying the effect of extract on the crystal size and antibacterial activity

Abstract

In this work, used the green method to preparing the silver nanoparticles (AgNPs) by using Citrus Limon Extract. The novel approach has been carried for the green synthesis of AgNPs by using different parts from Limon. Using X- RD to study the structural properties of samples and calculate the crystallite size then study the effect of deference parts of Citrus Limon plant.  appear from the results change in the crystallite size, that because the extract influence, and found changed depending on the type of extract (12.6,15.4,17.2 nm) when use (Seeds, Leaves, peels extract) respectively, also the results were analysis by use (AFM) atomic force microscopy, the results appear improving in structural properties by using the plant extract. Optical properties (Absorption and Transmittance) also investigated, appear dependence the optical properties of the prepared samples on the extract type, higher absorption when used seeds extract to preparing Ag Nps. Appeared from the results high antibacterial activity of AgNPs to both positive and negative gram. The inhibition zone of AgNPs depends on crystal size and the concentration of AgNPs, for that the inhibition zone radius increases with decrease the grain size. To evaluation the antibacterial activity of AgNPs against three kind of Gram-positive bacteria (E. faecalis, Staph.hominis and Staph.aureus), proved that Citrus Limon extract improved the performance of nanoparticles and found the highest inhibition diameter was 45 mm against Staph.hominis , wheen against types of Gram-negitive bacteria (Entero.cloacae,  E.coli and K.pneumoniae) found the highest inhibition zon 40mm againstStaph.hominis, wheen less inhibition zone 14 mm against E.coli.