Molecular Investigation of Some Genes Regulating the Production of Aflatoxin B1 in Wheat and Corn Grain

Authors

  • Olla Samer Alwan Ministry of Agriculture, Agriculture Extension Department, Iraq
  • Iqbal Harbi Mohammed Alzaidi Research Department, Horticulture Office, Ministry of Agriculture, Iraq
  • Shurook Saadedin University of Baghdad, Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, Baghdad, Iraq

DOI:

https://doi.org/10.54153/sjpas.2024.v6i3.748

Keywords:

aflatoxin B1 , afIR1, afIR2 Aspergillus flavus , , Aspergillus niger

Abstract

The research was conducted for the detection of aflatoxin B and toxin fungi associated with wheat and corn grain in Mysan. The samples were collected from markets and stores, with 50 samples of wheat and corn for each. Polymerase chain reaction technology has been used to rapidly detect aflatoxin B1 production of fungal isolates of a specific primer afIR1, afIR2. The results indicated the diagnosis of two types of fungus belonging to the genus Aspergillus, A.flavus, and A.niger, by 4% and 21%, respectively. Corn is the most polluted, with 72% and 28% of the wheat grain. The study showed that all isolates of. A.flavus was the carrier of the gene aflatoxin; for isolates of A.niger, the gene has appeared in three isolates but inconspicuously.

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Published

2024-09-30

How to Cite

Samer Alwan, O., Harbi Mohammed Alzaidi, I., & Saadedin, S. (2024). Molecular Investigation of Some Genes Regulating the Production of Aflatoxin B1 in Wheat and Corn Grain. Samarra Journal of Pure and Applied Science, 6(3), 236–244. https://doi.org/10.54153/sjpas.2024.v6i3.748

Issue

Vol. 6 No. 3 (2024): Samarra Journal of Pure and Applied Science

Section

Agriculture science

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