Effect Of Phosphate- Starvation On phoH In Acinetobacter baumannii
DOI:
https://doi.org/10.54153/sjpas.2025.v7i1.940Keywords:
Acinetobacter baumannii , inorganic phosphate, PhoH protein, q- PCR,Abstract
Acinetobacter baumannii is a Gram-negative bacteria that causes fatal bacterial infections and is resistant to environmental conditions such as lack of nutrients. Inorganic phosphate is considered one of the essential nutrients in bacterial cell metabolism. In our study, the effect of phosphate starvation on A.baumannii growth in M9 minimal medium, and on the expression of phoH (psiE) gene was determined.The expression level of phoH increased about 10 folds in the presence of K2HPO4 and about 5-fold with KH2PO4 in A. baumannii ATCC17904. Unlikely, in A. baumannii HHR1, phoH gene expression is remarkably induced with Na2HPO4, where the level of expression is about 25-folds higher than that of global strain. However, phoH increased by 3–fold with KH2PO4 in comparison to global strain. The growth of A. baumannii isolates in M9 medium exhibits different traits in the presence of inorganic phosphate sources, whereas the highest growth in global strainATCC17904 with Na2HPO4/NaH2PO4 together. While the local isolate exhibits the highest growth in the presence of NaH2PO4, whereas the eight clinical isolates showed growth variation in response to different sources. The clinically isolated A. baumannii L1, and L4 showed a higher growth rate with Na2HPO4, whereas L3 has the highest growth in K2HPO4/KH2PO4. L2, L5, L6, L7 and L8 reached a high growth rate with the combination of Na2HPO4/ NaH2PO4 , as for PhoH protein was around 41 kDa, and the band appeared when K2HPO4, KH2PO4, and Na2HPO4 were used, whereas, the band of PhoH protein was absent in the presence of NaH2PO4 and Na2HPO4/NaH2PO4 in HHR1 local strain.
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