Antagonism between carbon nano particles adsorption and virulence of pseudomonas aeruginosa

Abstract

The aqueous environment contamination by ppseudomonas hasbecome a great challenge for human beings. Walnut shells were washed by distilled water, mixed with KOH, burned at 650°C, and treated ultrasonically to obtain carbon nanoparticles. The bacterial isolates were from different sites of domestic water in hospitals and characteristic the pseudomonas. After that the minimum inhibitory concenttratio (MIC) was done to determine the optimum concentration that affected on p.aeruginosa and it was 0.006 mg/l , then using of polymerase chain reaction (PCR) to detect virulence factor genes (copB and czcA) and they characterized by biochemical and morphological diagnosis of p.aeruginosa in five isolates. Following treatment, the copB gene expression was 0.211 for isolate 2 and 0.047 for isolate 3, while the czcA gene expression was 0.882 for isolate 10 and 0.392 for isolate 11. The capacity of CNPs to reduce the gene expression in virulence factor genes was tested by using quantitative PCR (qPCR), as a result that affects bacteria, the CNPs at certain concentrations has high capacity to identify the virulence factors genes. These results were supported by XRD examination (Panalytical ‘X’ Pert Pr, UK), which indicated the presence of two Bragg diffraction peaks in the carbon nanoparticles, TEM (Zeiss LEO 912 AB/ Germany) results also showed the presence of inhomogeneous particles, as well as the irregular shape of the surface of the carbon nanoparticles with a large surface area, according to SEM examination (Zeiss Sigma VP/ Germany).