Detection of Biofilm forming bacteria causing Otitis media infection

Abstract

The current work aimed to isolate biofilm-forming bacteria causing otitis media infection and correlate with persistence and antibiotic resistance. In the beginning, sixty samples were gathered from patients who visited the ENT unit at Kirkuk General Hospital under the guidance of a medical professional. Samples were collected using cotton swabs from patients with chronic otitis media.  Bacterial diagnosis were performed using manual identification methods depending on Cultural characteristic's and biochemical tests. Antibiotic susceptibility tests was conducted using ten antibiotic discs following KirbyBauer method Biofilm production test was performed quantitatively using tissue microtiter plate method. The current results showed that several types of bacteria appeared, including Pseudomonas aeruginosa.  Staphylococcus aureus.  Staphylococcus epidermidis.  Escherishia. coli and Proteus vulgaris.  The antibiotics assay results showed that most isolates were resistant against Ceftazidim and Cefotaxime however, Imipenem gave good inhibitions against all recording 70% susceptibility. The biofilm production results included three types: Strong biofilm producer, Medium biofilm producer. Finally: Weak biofilm producer. The biofilm strength according to isolated bacteria included Pseudomonas aeruginosa. and S. aureus recording strong production while the weak production were recorded in E. coli and P. vulgaris. In conclusion, biofilm production is associated with drug resistant and recurrent or chronic ear infection.