Molecular Diagnosis with Study of the Antibiotics Susceptibility Pattern of Pathogenic Bacteria Isolated from Septicaemia Infections in Hospitals of Al-Ramadi City
DOI:
https://doi.org/10.54153/sjpas.2025.v7i1.897Keywords:
bacterial isolates, septicemia, molecular diagnosis, antibioticsAbstract
One hundred fifty samples of septicemia infections were collected for patients at the Women’s and Children’s Hospital and Ramadi General Teaching Hospital in the city of Ramadi (Anbar Governorate) during the period from 31/7/2023 to 1/11/2023. The samples were cultured on blood agar medium, chocolate agar medium, MacConkey agar medium, and mannitol salt agar medium, The results showed that 36 blood samples out of a total of 150 samples were culture positive, while 114 samples were culture negative, The results of the diagnosis of bacterial isolates indicated that the most common cause of septicemia is Staphylococcus spp. Bacteria, in the rate of 61% of the total bacterial isolates, Followed by Klebsiella pneumoniae bacteria at the rate of 28%, Then Pseudomonas aeruginosa bacteria at the rate of 11% of the total bacterial isolates, which is the lowest percentage in the incidence of infection of septicemia, The isolates were diagnosed using bacteriological and biochemical tests and the Vitek-2 device, and their diagnosis was confirmed molecularly using polymerase chain reaction (PCR) technology, In addition, the resistance to ten types of antibiotics was tested for the bacterial isolates under study using the disc diffusion method. The antibiotics included (Amikacin, Doxycilline, Augmentin, Linezolid, Imipenem, Levofloxacin, Trimemtheprim, Gentamicin, Rifampicin, and Aztreonam); the results of the resistance test showed Antibiotics have a significant difference in resistance and sensitivity rates among bacterial species.
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