Isolation and diagnosis of Klebsiella pneumoniae and evaluation of their resistance to modern antibiotics using the DL-120E diagnostic kit

Abstract

This study was conducted to evaluate the resistance of Klebsiella pneumoniae isolated from various infections to modern antibiotics. 140 samples were collected from various hospitals in Baghdad Governorate from various infections such as blood, urine, sputum, wounds, and burns from inpatients and outpatiants from both sexes and different ages from1/7/2023 to 2023/11/1. The samples included 65 samples from urinary tract infections, 35 samples from sputum 10 samples from blood and wound, and 20 samples from burns. The results showed that 96 samples, at a rate of 68.6%, showed bacterial growth. The study showed that the rate of infection in urine samples in females was greater than in males, at a rate of 28.1% for females, compared to males, which was 13.6%. As for sputum, blood, and it was at a rate of 31.2%, 4.1% respectively. As for wound and burns samples, it was 6.2%, 16.8%, respectively. The number of K.pneumoniae among other bacterial species was 20 isolates out of 96 samples. 8 from urine, 5 from sputum blood samples was 4, two from wound and one from burns. The results of the susceptibility of K.pneumoniaeto antibiotics using the DL-120E diagnostic kit was conducted. 26 antibiotics at different concentrations were used Meropenem and ertapenem were 100% susceptible. The highest rate of resistance was to the ampicillin at 95%, gentamicin 55%, ampicillin/sulbactam 75%, perracillin/tazobactam 55%, cefoperidol/sulbactam 50%, cefazolin 85%, cefepime 85%, and cefoxitin. 65%, cefuroxime 85%, levofloxase 60%, trimethoprim/sulfamethoxazole 70%, cefotaxime 80%, cefazidime 75%, and amikacin 55%.