Detection of the Staphylococcus aureus icA gene Isolated from some clinical cases

Abstract

One of the bacterial infections commonly found in the community, Staphylococcus aureus, is particularly important for its pathogenicity because of its virulence capacity to create biofilms. This study aimed to detect biofilm production in clinical isolates from wounds and urine and compare the levels of the gene icaA, 107 clinical sample from wounds and urine for Staphylococcus aureus isolation were taken from patients between November and December 2022 in Iraq at the Alzahra Clinic, Al-Kindi Teaching Hospital, and Alwasti Hospital. The biochemical test technique, the (Analytical Profile Index) API Staph system, the vitek 2 compact system, and finally, the polymerase chain reaction by detecting the 16SrRNA used to identified the S. aureus. S. aureus isolates were tested for their capacity to generate biofilm using 96-well microtiter.  a quantitative PCR assay was performed on 3 isolates with varying biofilm-building capacities. The results in this study show 29 isolated bacteria were identified as S. aureus. According to the results of biofilm study, 20.7% of the isolates produced weak biofilm, 13.8% produced strong biofilm, and 65.5% had a moderate capacity to produce biofilm. IcaA gene expression was high (6.508) in strong biofilm producer S. aureus. The strongest biofilm-producing strain exhibited significantly greater levels of gene expression in comparison to the weak and intermediate isolates.