Antimicrobial and antibiofilm activity of Biosynthesized Nickel Oxide Nanoparticles Using The culture supernatant of Staphylococcus aureus against Acenitobacter baumannii
DOI:
https://doi.org/10.54153/sjpas.2026.v8i1.1186Keywords:
antimicrobial activity, antibiofilm, Staphylococcus aureus, Acenitobacter baumannii, Nickel Oxide nanoparticles (NiO NPs).Abstract
The current study aimed to the synthesis of Nickel Oxide nanoparticles through the use of a biological method (using extracts of living organisms), as the extracted cellular product from Staphylococcus aureus was used as a catalyst for the reduction of nickel nitrate salt (Ni(NO3)2) to Nickel Oxide nanoparticles, in addition to the therapeutic applications of nanoparticles that will be used against MDR-A. baumannii bacteria, after characterizing these NiO nanoparticles using UV-VIS spectroscopy, AFM (Atomic Force Microscopy), XRD (X-ray diffraction), FE-SEM (field emission scanning electron microscopy), FTIR (fourier transform infrared) and EDX (Energy- Dispersive X-ray spectroscopy) and this is to understand the properties of NiO NPs.
The results revealed that Nickel Oxide nanoparticles with a nanosize of 63.81 nm were characterised by AFM microscopy and spherical shape under FE-SEM, the results also indicated the identification of MDR-A.baumannii isolates possessing high resistance In the following study and antibacterial activity test against MDR-A.baumannii was carried out against MDR-A.baumannii using different concentrations of Nickel Oxide nanoparticles (500 µg/ml, 1 mg/ml, 2 mg/ml and 4 mg/ml), the results of the in vitro evaluation of bacterial biosynthesised Nickel Oxide nanoparticles as antibacterial showed that the Maximum Inhibitory Concentration (MIC) was 4 mg/ml with an area diameter of 22 mm, while the Minimum Inhibitory Concentration (MIC) was 2 mg/ml with an area diameter of 18 mm, the biosynthesis of Nickel nanoparticles showed promising antibacterial activity.
The eradication of biofilms using NIO NPs in multidrug-resistant bacteria MDR-A.baumannii at concentrations of 4 ml/gm and 8 ml/gm, respectively, against biofilm production, showed that nickel nanoparticles had a significant antibacterial and biofilm inhibitory effect, which could have applications as a treatment against multidrug resistant MDR-A.baumannii bacteria.
References
1. Gharaibeh, M. H., Abandeh, Y. M., Elnasser, Z. A., Lafi, S. Q., Obeidat, H. M., & Khanfar, M. A. (2024). Multi-drug resistant Acinetobacter baumannii: phenotypic and genotypic resistance profiles and the associated risk factors in teaching hospital in Jordan. J Infect Public Health, 17(4), 543-550.
2. Hamidian, M., & Nigro, S. J. (2019). Emergence, molecular mechanisms and global spread of carbapenem-resistant Acinetobacter baumannii. Microbial genomics, 5(10), e000306.
3. Al-Tamimi, M., Albalawi, H., Alkhawaldeh, M., Alazzam, A., Ramadan, H., Altalalwah, M., Alma'aitah, A., Albalawi, D., Shalabi, S., Abu-Raideh, J., Khasawneh, A., Alhaj, F. and Hijawi, K. (2022). Multidrug-Resistant Acinetobacter baumannii in Jordan, Microorganisms, 10, 849.
4. Mohammed, S. H. (2022). Prevalence of Acinetobacter Spp. Isolated from Clinical Samples Referred to Al- Kafeel Hospital and Their Antibiotic Susceptibility Patterns from 2017-2021, Iran J Med Microbiol, 16(1): 76-82.
5. Kim, U. J., Kim, H. K., An, J. H., Cho, S. K., Park, K. H., & Jang, H. C. (2014). Update on the epidemiology, treatment, and outcomes of carbapenem-resistant Acinetobacter infections. Chonnam medical journal, 50(2), 37-44.
6. Al-Sheboul, S. A., Al-Moghrabi, S. Z., Shboul, Y., Atawneh, F., Sharie, A. H., & Nimri, L. F. (2022). Molecular characterization of carbapenem-resistant Acinetobacter baumannii isolated from intensive care unit patients in Jordanian hospitals. Antibiotics, 11(7), 835.
7. Schulze, A., Mitterer, F., Pombo, J. and Schild, S. (2021). “Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies”. Microbial Cell, vol. 8, no. 2, pp. 28-56.
8. Badave, G. K., and Kulkarni, D. (2015). Biofilm producing multidrug resistant Acinetobacter baumannii: an emerging challenge. Journal of clinical and diagnostic research: JCDR, 9(1), DC08.
9. Tarín-Pelló, A., Suay-García, B., & Pérez-Gracia, M. T. (2022). Antibiotic resistant bacteria: current situation and treatment options to accelerate the development of a new antimicrobial arsenal. Expert Review of Anti-infective Therapy, 20(8), 1095-1108.
10. Chaudhary, J., Tailor, G., Yadav, B. L. & Michael, O. (2019). Synthesis and biological function of nickel and copper nanoparticles. Heliyon 5, e01878.
11. El-Kemary, M., Nagy, N., and El-Mehasseb, I. (2013).“Nickel oxide nanoparticles: Synthesis and spectral studies of interactions with glucose”. Materials Science in Semiconductor Processing, vol.16, no.6, pp. 1747-1752.
12. Yaaqoob, L. A., Abed, R. M., Kamona, Z. K., Altaee, M. F. and Younis, R. W. (2022).“Evaluation the ability of titanum oxide nanoparticles to increase the production of prodigiosin and phenasine from serratia marcescens and pseudomonas aeruginosa respectively”. Iraqi Journal of Agricultural Sciences. vol. 53, no. 3, pp.496- 504.
13. Kumari, S. C., Dhand, V. & Padma, P. N. (2021). Green synthesis of metallic nanoparticles: A review. In Nanomaterials 259–281.
14. Ahmad, B. et al.(2022). Green synthesis of NiO nanoparticles using aloe vera gel extract and evaluation of antimicrobial activity. Mater. Chem. Phys. 288, 126363.
15. Ahghari, M. R., Soltaninejad, V. & Maleki, A. (2020). Synthesis of nickel nanoparticles by a green and convenient method as a magnetic mirror with antibacterial activities. Sci. Rep. 10, 1–10.
16. Sabouri, Z., Akbari, A., Hosseini, H. A., Khatami, M. & Darroudi, M. (2021). Green-Based bio-synthesis of nickel oxide nanoparticles in Arabic gum and examination of their cytotoxicity, photocatalytic and antibacterial effects. Green Chem. Lett. Rev. 14, 402–412.
17. Din, S. U., Iqbal, H., Haq, S., Ahmad, P., Khandaker, M. U., Elansary, H. O., ... & El-Abedin, T. K. Z. (2022). Investigation of the biological applications of biosynthesized nickel oxide nanoparticles mediated by Buxus wallichiana extract. Crystals, 12(2), 146.
18. Al-Zaqri, N., Umamakeshvari, K., Mohana, V., Muthuvel, A. & Boshaala, A. (2022). Green synthesis of nickel oxide nanoparticles and its photocatalytic degradation and antibacterial activity. J. Mater. Sci. 33, 11864–11880.
19. Ghayyib, A. A. (2022). Isolation and Characterization of Staphylococcus aureus Bacteriophage and Evaluation of Its Antibacterial Activity (Doctoral dissertation, Ministry of Higher Education).
20. American Society for Microbiology. 98th General Meeting Workshop Program. (1998). Practical Approach to the Identification of the Medically Important Glucose Non-Fermenting Gram-Negative Bacilli. American Society for Microbiology, Washington, D.C.
21. Gurjar, K. C., Agrawal, A., Kumar, S., Sharma, R., Pandey, K., Pandey, H., & Awasthi, A. (2023). Antimicrobial efficacy of Ag-doped ZnO nanocomposite against Bacillus subtilis. Materials Today: Proceedings, 95, 61-66.
22. Maruthupandy, M., Rajivgandhi, G. N., Quero, F., & Li, W. J. (2020). Anti-quorum sensing and anti-biofilm activity of nickel oxide nanoparticles against Pseudomonas aeruginosa. Journal of Environmental Chemical Engineering, 8(6), 104533.
23. Bahjat, H. H., Ismail, R. A., Sulaiman, G. M., and Jabir, M. S. (2021). Magnetic field-assisted laser ablation of titanium dioxide nanoparticles in water for anti-bacterial applications. Journal of Inorganic and Organometallic Polymers and Materials, 1-8.
24. Al Rugaie, O., Jabir, M. S., Mohammed, M. K., Abbas, R. H., Ahmed, D. S., Sulaiman, G. M., ... & Mohammed, H. A. (2022). Modification of SWCNTs with hybrid materials ZnO–Ag and ZnO–Au for enhancing bactericidal activity of phagocytic cells against Escherichia coli through NOX2 pathway. Scientific Reports, 12(1), 17203.
25. Jassim, S. M., Abd, M. A., & Hammed, I. A. (2023). Green synthesis of nickel oxide nanoparticles using syzygium aromatic extract: characterization and biological applications. Al-Bahir Journal for Engineering and Pure Sciences, 2(2), 7.
26. Al-taee, M. F., Yaaqoob, L. A., & Kamona, Z. K. (2020). Evaluation of the Biological Activity of Nickel Oxide Nanoparticles as Antibacterial and Anticancer Agents. Iraqi Journal of Science, 2888-2896.
27. Lingaraju, K., Naika, H. R., Nagabhushana, H., Jayanna, K., Devaraja, S., & Nagaraju, G. (2020). Biosynthesis of nickel oxide nanoparticles from Euphorbia heterophylla (L.) and their biological application. Arabian Journal of Chemistry, 13(3), 4712-4719.
28. Rajesh, A. S., Kumar, Kishore, N., Budhiraja, N. (2013). Preparation and characterization of NiO nanoparticles by co-precipitation method Inter. J. Eng. Appl. Manag. Sci. Para., 06, pp. 64-67
29. Mahdavi, M., Ahmad, M. B., Haron, M. J., Namvar, F., Nadi, B., Rahman, M. Z. A. and Amin, J. (2013). “Synthesis, surface modification and characterization of biocompatible magnetic iron oxide nanoparticles for biomedical applications”. Molecules, vol. 18, no. 7, pp. 7533-7548.
30. Saleem, S., Ahmed, B., Khan, M., Shaeri, A. I. and Musarrat, J. (2017). “Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants”. Microbial Pathogenesis, vol. 111, pp. 375-387.
31. Hammood, I. A., Nijris, O. N., & Alwan, L. H. (2022). Comparing the effect of zinc oxide nanoparticles biosynthesized by Aloe barbdensis and chemically manufactured on Klebsiella pneumoniae isolated from the inflamed middle ear. International journal of health sciences, 6(S2), 14976-14988.
32. Chen, Y., Feng, N., Ji, Q., Zhang, Y., Li, W. and Wang, J. (2021). “Space Environment and Charge Characteristics of Flexible Secondsurface Mirrors Gaodianya Jishu”. High Voltage Engineering, vol. 47, no. 7, pp. 201-255.
33. Khalil, M. A., El Maghraby, G. M., Sonbol, F. I., Allam, N. G., Ateya, P. S., & Ali, S. S. (2021). Enhanced efficacy of some antibiotics in presence of silver nanoparticles against multidrug resistant Pseudomonas aeruginosa recovered from burn wound infections. Frontiers in microbiology, 12, 648560.
34. Peulen, T. O., & Wilkinson, K. J. (2011). Diffusion of nanoparticles in a biofilm. Environmental science & technology, 45(8), 3367-3373.
35. Yaseen, A. H., & Yaaqoob, L. A. (2023). Biosynthesis of cobalt oxide nanoparticles using blood serum as reducing and stabilizing agent for therapeutic use against multi-drug resistant Acinetobacter baumannii
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