Targeted delivery preparation anti-bacterial drug liposomal levofloxacin and determination by a high-performance liquid chromatography

Abstract

A new method for evaluation and preparation of liposomal levofloxacin by using a modified method of thin film hydration to prepare the liposomal drug, Traditional lipids such as phosphatidylcholine and beta sitosterol was used for the preparation of the liposomal drug, and the drug was evaluated and characterized the shape and dimension using scanning electron microscopy (SEM), the method was providing spherical shape and provide dimension 17.18 μm and the stability of liposomal drug was studied by zeta potential and it was – 56.46 mV. That provides a high stability and mobility of the liposomal drug in the suspension solution without precipitating the pellets, additionally the liposomal levofloxacin was evaluated using RP-HPLC and demonstrating very good separation and provided a high recovery and the relative coefficient was R² 0.9999 of the concentration range (0.00002-0.04) mmole/ml and the LOD and LOQ were 1.04×10^-8 mmole and 3.47×10^-8 mmole respectively. The entrapment efficiency was studied for the liposomal drug to ensure the method used for the preparation of liposomal levofloxacin and evaluated and it was 97.3%.