Evaluation of the antibacterial effect of ciprofloxacin coated with silver nanoparticles (AgNPs) on pseudomonas aeruginosa isolated from wounds

Abstract

  Pseudomonas aeruginosa is an opportunistic pathogen commonly associated with burn wound infections and known for its resistance to multiple antibiotics. In this study, ciprofloxacin was combined with biosynthesized silver nanoparticles (AgNPs) derived from Thymus capitatus leaf extract to evaluate their antibacterial efficacy against Ps. aeruginosa. Clinical isolates were obtained from burn patients and identified using morphological and biochemical methods. Silver nanoparticles were synthesized via green synthesis, characterized by X-Ray Diffraction) XRD(, Fourier Transform Infrared Spectroscopy) FTIR(, Energy Dispersive X-ray Spectroscopy) EDX(, Scanning Electron Microscopy) SEM(, and Transmission Electron Microscopy) TEM( analyses, and confirmed to be predominantly spherical. The antibacterial activity was evaluated using the agar diffusion method. Results demonstrated that AgNPs alone exhibited a strong inhibitory effect, with a maximum inhibition zone of 18.67 ± 0.58 mm at 64 µg/mL, whereas ciprofloxacin alone showed an inhibition zone of 14.00 ± 1.00 mm. The combination of ciprofloxacin with silver nanoparticles) AgNPs(slightly enhanced the antibacterial activity (15.33±0.58 mm), indicating a synergistic effect. These findings suggest that silver nanoparticles) AgNPs( have significant potential as an adjuvant to conventional antibiotics for combating multidrug-resistant Psedomonas aeruginosa in wound infections.