Immunostimulatory and anti-inflammatory effects of Pleuran purified from the edible mushroom Pleurotus ostreatus var. ostreatus in the hydrogen peroxide-induced inflammation in white rats

Abstract

This study was conducted at the laboratories of the College of Agriculture and Animal House in the college of Veterinary Medicine, Tikrit University, during 2022/2023, with the aim of evaluating the effect of Pleuran purified from the edible mushroom Pleurotus ostreatus var. ostreatus as immunomodulatory and antioxidant agent in the Inflammatory rats with Hydrogen peroxide. The results of the Pleuran effect In vivo (white rats) showed that all Pleuran concentrations did not have a negative impact on the hematological parameters in healthy animals. However, these treatments led to improvements in the white blood cell counts, restoring them to normal levels in animals induced with inflammation by hydrogen peroxide, the highest improvements were observed at 15 mg/kg body weight of Pleuran, with white blood cell and platelet counts reaching 7500 and 188000 cells/μL,respectively. Neutrophil/lymphocyte index decreased from 2.58 in the Inflammatory animals to 1.12 in the presence of Pleuran at 15 mg/kg/day, compared to 1.3 in the levamisole group. The lowest immunoglobulin G (IgG) and M (IgM) was 406.3 and 45.3 mg/dL in the hydrogen peroxide treatment alone, then increased in all pleuran concentration, the highest values were 746.3 and 118.3 mg/dL in pleuran at 15 mg/kg/day,  compared  to 585.3 and 90.3 mg/dL in the levamisole group, while the results showed increase in  Tumor Necrosis Factor-Alpha (TNF-α)  and Interleukin-6 (IL-6) to 103.8 pg/ml  and 37.8 ng/L in the hydrogen peroxide treatment alone, and increased in all pleuran concentration, with highest values 68.4 pg/ml  and 9.66 ng/L in present of pleuran at 15 mg/kg/day, compared  to 89.8 pg/ml  and 9.66 ng/L in the levamisole group, respectively.