التأثير الأيضي للمنتج الثانوي لـ Saccharomyces ellipsoideus ضد بعض الأنواع البكتيرية المهمة طبياً من وسائط النمو التقليدية والمعدلة
DOI:
https://doi.org/10.54153/sjpas.2025.v7i1.1139الكلمات المفتاحية:
Shifted modified Chebyshev polynomials, Optimal control problem, Operational matrix, Numerical examples, Orthogonal function.الملخص
Saccharomyces ellipsoideus هو نوع من الخميرة مع نواتج أيضية تعزز مقاومة الحموضة وتعمل كعامل مضاد للالتهابات وتحفز الجهاز المناعي. بالإضافة إلى ذلك، يُظهر S. ellipsoideus تأثيرات مضادة للميكروبات ضد مسببات الأمراض البكتيرية المعوية. تهدف هذه الدراسة إلى تقييم تأثير المنتجات الأيضية الثانوية المشتقة من المغذيات والوسائط المعدلة لـ S. ellipsoideus على أنواع بكتيرية مختلفة. الطرق: تم الحصول على خميرة S. ellipsoideus من الأسواق المحلية في مدينة الرمادي، لاختبارها ضد نمو الأنواع البكتيرية بما في ذلك Enterococcus faecalis و Saphylococcus epidermidis و Escherichia coli و Serratia marcescens و Pseudomonas aeruginosa. تم اختبار حساسية البكتيريا لنواتج أيض S. ellipsoideus باستخدام طريقة انتشار الأجار. النتائج: تشير نتائج الدراسة إلى أن S. ellipsoideus المزروعة في بيئات معدلة أظهرت نشاطًا مضادًا للميكروبات متزايدًا ضد الأنواع البكتيرية الخمسة بمرور الوقت. لوحظت التأثيرات المضادة للميكروبات الأكثر أهمية ضد E. coli و P. aeruginosa و S. marcescens، وخاصة بعد فترة حضانة مدتها 168 ساعة، حيث تم تسجيل مناطق تثبيط 19 مم و 18 مم على التوالي. الاستنتاج: خلصت الدراسة إلى أن S. ellipsoides يثبط بشكل فعال نمو خمسة أنواع من البكتيريا. علاوة على ذلك، تم تعزيز النشاط المضاد للميكروبات لـ S. ellipsoides بشكل ملحوظ عند نموه في بيئات معدلة مقارنة بالوسائط المغذية.
المراجع
1. Souza, H. F. D., Carosia, M. F., Pinheiro, C., Carvalho, M. V. D., Oliveira, C. A. F. D., & Kamimura, E. S. (2021). On probiotic yeasts in food development: Saccharomyces boulardii, a trend. Food Science and Technology, 42, e92321. https://doi.org/10.1590/fst.92321
2. Hadjimbei, E., Botsaris, G., Goulas, V., Alexandri, E., Gekas, V., & Gerothanassis, I. P. (2020). Functional stability of goats' milk yoghurt supplemented with Pistacia atlantica resin extracts and Saccharomyces boulardii. International Journal of Dairy Technology, 73(1), 134-143. https://doi.org/10.1111/1471-0307.12629
3. Tao, Z., Yuan, H., Liu, M., Liu, Q., Zhang, S., Liu, H., ... & Wang, T. (2023). Yeast extract: characteristics, production, applications and future perspectives. Journal of microbiology and biotechnology, 33(2), 151. https://doi.org/10.4014%2Fjmb.2207.07057
4. Moslehi-Jenabian, S., Pedersen, L. L., & Jespersen, L. (2010). Beneficial effects of probiotic and food borne yeasts on human health. Nutrients, 2(4), 449-473. https://doi.org/10.3390/nu2040449
5. Egea, M. B., Oliveira Filho, J. G. D., & Lemes, A. C. (2023). Investigating the Efficacy of Saccharomyces boulardii in Metabolic Syndrome Treatment: A Narrative Review of What Is Known So Far. International Journal of Molecular Sciences, 24(15), 12015. https://doi.org/10.3390/ijms241512015
6. Fu, J., Liu, J., Wen, X., Zhang, G., Cai, J., Qiao, Z., ... & Li, L. (2023). Unique probiotic properties and bioactive metabolites of Saccharomyces boulardii. Probiotics and Antimicrobial Proteins, 15(4), 967-982.
7. McFarland, L. V. (2017). Common organisms and probiotics: Saccharomyces boulardii. In The microbiota in gastrointestinal pathophysiology (pp. 145-164). Academic Press. https://doi.org/10.1016/B978-0-12-804024-9.00018-5
8. Bagherpour, G., Ghasemi, H., Zand, B., Zarei, N., Roohvand, F., Ardakani, E. M., ... & Khalaj, V. (2018). Oral administration of recombinant Saccharomyces boulardii expressing ovalbumin-CPE fusion protein induces antibody response in mice. Frontiers in microbiology, 9, 723. https://doi.org/10.3389/fmicb.2018.00723
9. Goktas, H., Dertli, E., & Sagdic, O. (2021). Comparison of functional characteristics of distinct Saccharomyces boulardii strains isolated from commercial food supplements. LWT, 136, 110340. https://doi.org/10.1016/j.lwt.2020.110340
10. Tomičić, Z. M., Čolović, R. R., Čabarkapa, I. S., Vukmirović, Đ. M., Đuragić, O. M., & Tomičić, R. M. (2016). Beneficial properties of probiotic yeast Saccharomyces boulardii. Food and Feed Research, 43(2), 103-110. https://doi.org/10.5937/FFR1602103T
11. Kaźmierczak-Siedlecka, K., Ruszkowski, J., Fic, M., Folwarski, M., & Makarewicz, W. (2020). Saccharomyces boulardii CNCM I-745: a non-bacterial microorganism used as probiotic agent in supporting treatment of selected diseases. Current microbiology, 77, 1987-1996. https://doi.org/10.1007/s00284-020-02053-9
12. Durmusoglu, D., Al’Abri, I., Li, Z., Islam Williams, T., Collins, L. B., Martínez, J. L., & Crook, N. (2023). Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach. Microbial Cell Factories, 22(1), 109. https://doi.org/10.1186/s12934-023-02117-y
13. Isayenko, O. Y., Knysh, O. V., Babych, Y. M., Ryzhkova, T. N., & Dyukareva, G. I. (2019). Effect of disintegrates and metabolites of Lactobacillus rhamnosus and Saccharomyces boulardii on biofilms of antibiotic resistant conditionally pathogenic and pathogenic bacteria. Regulatory Mechanisms in Biosystems, 10(1), 3-8. https://doi.org/10.15421/021901
14. Deak, T., & Beuchat, R. (1987). Identification of food borne yeasts. J. Food Protect. 50, 243-264.
15. Barnett, J. A., Payne, R. W., & Yarrow, D. (1990). Yeasts: characteristics and identification.
16. AL Zubaidy, Z. M & Khidhr, K. O. (2014). Isolation and Identification of Saccharomyces cerevisiae var boulardii and its Uses as a Probiotic (in vitro). Rafidain journal of science, 25(1), 1-11.
17. Hossain, M. N., Afrin, S., Humayun, S., Ahmed, M. M., & Saha, B. K. (2020). Identification and growth characterization of a novel strain of Saccharomyces boulardii isolated from soya paste. Frontiers in nutrition, 7, 27. https://doi.org/10.3389%2Ffnut.2020.00027
18. Attaie, R., Whalen, P. J., & Shahani, K. M. (1987). Inhibition of Growth of Staphylococcus aureus during Production of Acidophilus Yogurt. Journal of Food Protection, 50(3), 224-228.
19. Drew WL, Barry A, O'Toole R, Sherris JC. Reliability of the Kirby-Bauer disc diffusion method for detecting methicillin-resistant strains of Staphylococcus aureus. Applied microbiology. 1972;24(2):240-247.
20. Dolka, B., Czopowicz, M., Chrobak-Chmiel, D., Ledwoń, A., & Szeleszczuk, P. (2020). Prevalence, antibiotic susceptibility and virulence factors of Enterococcus species in racing pigeons (Columba livia f. domestica). BMC veterinary research, 16, 1-14. https://doi.org/10.1186%2Fs12917-019-2200-6
21. Janezic, K. J., Ferry, B., Hendricks, E. W., Janiga, B. A., Johnson, T., Murphy, S., ... & Daniel, S. L. (2013). Phenotypic and genotypic characterization of Escherichia coli isolated from untreated surface waters. The open microbiology journal, 7, 9. https://doi.org/10.2174%2F1874285801307010009
22. Monteiro, A. C. M., Fortaleza, C. M. C. B., Ferreira, A. M., Cavalcante, R. D. S., Mondelli, A. L., Bagagli, E., & da Cunha, M. D. L. R. D. S. (2016). Comparison of methods for the identification of microorganisms isolated from blood cultures. Annals of clinical microbiology and antimicrobials, 15, 1-11. https://doi.org/10.1186/s12941-016-0158-9
23. Pradhan, P., & Tamang, J. P. (2019). Phenotypic and genotypic identification of bacteria isolated from traditionally prepared dry starters of the Eastern Himalayas. Frontiers in Microbiology, 10, 492547. https://doi.org/10.3389/fmicb.2019.02526
24. Alves, M., Ferreira, I. F. R., Dias, J., Teixeira, V., Martins, A., & Pintado, M. (2012). A review on antimicrobial activity of mushroom (basidiomycetes) extracts and isolated compounds. Planta Medica, 78(16), 1707–1718. https://doi.org/10.1055/s-0032-1315370
25. Miller, W. R., Munita, J. M., & Arias, C. A. (2014). Mechanisms of antibiotic resistance in enterococci. Expert review of anti-infective therapy, 12(10), 1221-1236.. https://doi.org/10.1586/14787210.2014.956092
26. Rajkowska, K., Kunicka-Styczynska, A., & Rygala, A. (2012). Probiotic activity of Saccharomyces cerevisiae var. boulardii against human pathogens. Food Technology and Biotechnology, 50(2), 230.
27. Jalal, R. A., & Aziz, K. E. (2023). Usage of Antimicrobial Activity Against E. Coli O157:H7 Isolated From Local Meat and Vegetables in Erbil City. Anbar Journal of Agricultural Sciences, 21(1), 114–123. https://doi.org/10.32649/ajas.2023.179721
28. Ali, M. A. E., Abdel-Fatah, O. M., Janson, J. C., & Elshafei, A. M. (2012). Antimicrobial potential of Saccharomyces boulardii extracts and fractions. Journal of Applied Sciences Research, 8(8), 4537– 4543.
29. Canani, R. B., Cucchiara, S., Cuomo, R., Pace, F., & Papale, F. (2011). Saccharomyces boulardii: a summary of the evidence for gastroenterology clinical practice in adults and children. Eur Rev Med Pharmacol Sci, 15(7), 809-822.
30. Venkateswarulu, T. C., Srirama, K., Mikkili, I., Nazneen Bobby, M., Dulla, J. B., & Alugunulla, V. N. (2019). Estimation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of antimicrobial peptides of Saccharomyces boulardii against selected pathogenic strains. Karbala Int J Mod Sci, 5(4), 266-9. https://doi.org/10.33640/2405-609X.1219
31. Hyrslova, I., Kana, A., Nesporova, V., Mrvikova, I., Doulgeraki, A. I., Lampova, B., ... & Krausova, G. (2024). In vitro digestion and characterization of selenized Saccharomyces cerevisiae, Pichia fermentans and probiotic Saccharomyces boulardii. Journal of Trace Elements in Medicine and Biology, 83, 127402. https://doi.org/10.1016/j.jtemb.2024.127402
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