Determination The Drug Adrenaline in Pharmaceutical Forms By Spectrophotometric Method Using N-Bromo succinimide and p-Anisidine Reagents

Abstract

In order to determine the amount of adrenaline [ADR] in pure and pharmaceutical formulations, this research attempts to create an indirect, easy to understand sensitive, and cost-effective spectrophotometric approach. The suggested technique is predicated on the oxidation of ADR in an acidic medium with a certain amount of N-bromosuccinimide [NBS]. After oxidation is complete, the remaining NBS reacts with the reagent p-Anisidine, resulting in a colored brominated product with the maximum absorbance at 516 nm, which is inversely proportional to the amount of ADR in the sample. Plotting the method's standard curve revealed a linear relationship with a determination coefficient of 0.9995 for the concentration range of 1.0–12.0 μg/mL. The Sandell sensitivity index was 0.0203 μg/cm², and the molar absorption coefficient was 9.01 × 10³ L/mol.cm. The quantification [LOQ] and detection [LOD] limits were 0.493 and 0.178 μg/mL, respectively. With respectable accuracy and sensitivity, this approach has been effectively used to estimate adverse drug effects in pharmaceutical preparations and human blood serum.