Determination The Drug Adrenaline in Pharmaceutical Forms By Spectrophotometric Method Using N-Bromo succinimide and p-Anisidine Reagents
DOI:
https://doi.org/10.54153/sjpas.2026.v8i2.1296Keywords:
Spectrophotometric, Adrenaline, Determination, N-bromosuccinimide, p-AnisidineAbstract
In order to determine the amount of adrenaline [ADR] in pure and pharmaceutical formulations, this research attempts to create an indirect, easy to understand sensitive, and cost-effective spectrophotometric approach. The suggested technique is predicated on the oxidation of ADR in an acidic medium with a certain amount of N-bromosuccinimide [NBS]. After oxidation is complete, the remaining NBS reacts with the reagent p-Anisidine, resulting in a colored brominated product with the maximum absorbance at 516 nm, which is inversely proportional to the amount of ADR in the sample. Plotting the method's standard curve revealed a linear relationship with a determination coefficient of 0.9995 for the concentration range of 1.0–12.0 μg/mL. The Sandell sensitivity index was 0.0203 μg/cm2, and the molar absorption coefficient was 9.01 × 103 L/mol.cm. The quantification [LOQ] and detection [LOD] limits were 0.493 and 0.178 μg/mL, respectively. With respectable accuracy and sensitivity, this approach has been effectively used to estimate adverse drug effects in pharmaceutical preparations and human blood serum.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Notice
Authors retain copyright and grant the SJPAS journal right of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in Samarra Journal of Pure and Applied Science.
The Samarra Journal of Pure and Applied Science permits and encourages authors to archive Pre-print and Post-print items submitted to the journal on personal websites or institutional repositories per the author's choice while providing bibliographic details that credit their submission, and publication in this journal. This includes the archiving of a submitted version, an accepted version, or a published version without any Risks.
