Detection of Interleukin-8, Interleukin-10 and Tumor Necrosis Factor alpha (TNF- α) among pregnant women infected with acute Trichomoniasis

Abstract

Trichomoniasis is one of the most common sexually transmitted diseases caused by Trichomonas vaginalis parasite, affecting females in particular at different ages. This study was conducted between September 2023 and March 2024 at Al-Batool Teaching Hospital for gynecological examination. A total of fifty high vaginal swab (HVS) and serum samples were collected from symptomatic women of various ages presenting with vaginal itching. High vaginal swabs were obtained from each women and diagnosed  microscopically then immunological test is performed by using the enzyme-linked immunosorbent assay (ELISA) technique.  The present study shows that of the total 50 patients, 21 (42%) were infected with Trichomonas vaginalis, and 29 (58%) were negative for Trichomonas vaginalis. Further, there was a significant increase in the mean of IgM, IgG, MMP-9 and  TNF-α in infected women with Trichomonas vaginalis that were (8.7±3.1 ng/dl, 9.01±1.90 ng/dl, 5.01 ± 1.74 ng/ml, and (145.808 + 1.81) pg/ml,  respectively, as compared with control. While there were no differences in IL-8 and IL-10 levels in infected women compared control. This study concluded increase IgM , IgG, mmp-9, and TNF-α in infected women with Trichomonas vaginalis, while no differences in IL-8, IL10.